An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media.
نویسندگان
چکیده
To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.
منابع مشابه
Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry
OBJECTIVE An experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry. METHODS An anaerobic digestate sample was collected from an anaerobic digester using pig slurry. Pre-reduced media were used for the growth and isolation of methanogens. Growth temperature range, pH range, NaCl concentration range, substrate utilization, and ...
متن کاملState of Iron in the Archaebacterium Methanosarcina barkeri Grown on Different Carbon Sources as Studied by Mössbauer Spectroscopy
Archaebacterium, Methanosarcina, Methanol, Iron, Mössbauer, Ferredoxin Cells of the archaebacterium Methanosarcina barkeri were grown strictly anaerobic in defined media. 57Fe-Mössbauer Spectroscopy of methanol grown cells oxidized in the presence of air demonstrated only the state of Fe3+ whereas active cells under reducing conditions offered spectra of two additional iron sites in the Fe2+ st...
متن کاملUsing Insects\' Body Powder as a Complementary Growth Factor in Fungal Culture Media
Introduction: Nutritional requirements of fungi are essential for their successful growth in laboratories. Some fungi feed on the compounds present in the insects' bodies. The aim of this study was to evaluate the effect of insects' body powder (IBP) as a supplement for the fungi growth in culture media. Methods: We used 10 mg of IBP, obtained from Anopheles superpictus and Culex theileri mosqu...
متن کاملEffect of Nickel Levels on Hydrogen Partial Pressure and Methane Production in Methanogens
Hydrogen (H2) consumption and methane (CH4) production in pure cultures of three different methanogens were investigated during cultivation with 0, 0.2 and 4.21 μM added nickel (Ni). The results showed that the level of dissolved Ni in the anaerobic growth medium did not notably affect CH4 production in the cytochrome-free methanogenic species Methanobacterium bryantii and Methanoculleus bourge...
متن کاملPii: S0043-1354(00)00438-3
Microbial population dynamics were evaluated in anaerobic codigesters treating municipal solid waste and sewage sludge. Ribosomal RNA based oligonucleotide probes were used to characterize changes in population abundance of syntrophic volatile fatty acid degrading bacteria and methanogens. Changes in community structure were linked to traditional performance parameters during the recovery of pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 64 2 شماره
صفحات -
تاریخ انتشار 1998